3,5-difluoro-4-hydroxybenzylidene imidazolinone-2-oxime (DFHO)










4-(3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime / 1420815-34-4


Fluorescence Spectra



A. Absorption and fluorescence emission spectra of CornTM/DFHO complex. B. Absorption and fluorescence emission spectra of Red and Orange BroccoliTM/DFHO complex.


PRESENTATION: Lyophilized dye


STORAGE CONDITIONS: Stable in the dark for 2 year at -20 ºC.


QUALITY ASSURANCE: Products are analyzed by 1H NMR and LC-MS and provided at purity of >95% by HPLC.


USAGE STATEMENT: This product is intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Due to the highly specific nature of fluorophores and aptamers, we cannot predict or be held responsible with respect to how LucernaTM products will behave in its customers’ systems. Researchers using LucernaTM products should conduct optimization studies to achieve the optimal result possible for their intended application.





A. Live-cell imaging of HEK293T cells expressing  U6 promoter driven CornTM in the presence of 10 µM DFHO.  Images are Hoechst-stained nuclei (blue) and CornTM fluorescence (yellow), individually or overlayed.  Images were acquired using YFP and DAPI filter sets.  B. Live-cell imaging of E. coli cells expressing tRNA promoter driven CornTM in the presence of 10 µM DFHO.  



Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Song W, Filonov GS, Kim H, Hirsch M, Li X, Moon JD, Jaffrey SRNat Chem Biol. 2017 Nov;13(11):1187-1194.


A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Warner KD, Sjekloca L, Song W, Filonov GS, Jaffrey SR, Ferre-D’Amare ARNat Chem Biol. 2017 Nov;13(11):1195-1201.


Spectral tuning by a single nucleotide controls the fluorescence properties of a fluorogenic aptamer. Filonov GS, Song W, Jaffrey SR. Biochemistry. 2019 Mar 26;58(12):1560-1564.


This Product Was Cited In