BI is a DFHBI derivative that exhibits unprecedented Broccoli™ binding affinity and photostability, and it can be used for single-molecule mRNA imaging in live mammalian cells.
3’5′-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) is a small molecule mimic of the GFP chromophore. It is highly fluorescent upon binding to Spinach and Spinach-derived aptamers.
DFHBI-1T is a non-fluorescent dye that consists of an 1,1,1-trifluoroethyl substituent on the imidazolone ring of the DFHBI, a mimic of GFP fluorophore.
DFHO is a non-fluorescent dye that is structurally similar to the chromophore found in red fluorescent protein (RFP).
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